| ALX-804-015-C100 | 100 µg | 801.00 USD |  |
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| Alternative Name: | RyR |
| Clone: | C3-33 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | Dog cardiac RyR (ryanodine receptor). |
| UniProt ID: | Q92736 (human RYR2) |
| Species reactivity: | Human, Mouse, Rat Amphibian, Chicken, Dog, Fish, Guinea pig, Rabbit |
| Crossreactivity: | Cross-reacts weakly with RyR-1. |
| Applications: | Flow Cytometry, ICC, IHC (FS), IHC (PS), IP, WB |
| Recommended Dilutions/Conditions: | Immunocytochemistry (1:100-1:200) Immunohistochemistry (frozen sections, 1µg/ml) Immunohistochemistry (paraffin sections, 1µg/ml) Western Blot (1µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Application Notes: | Detects a band of 565kDa by Western blot. In non-mammalian vertebrates, a doublet is seen at 565kDa representing the α and β isoforms of the receptor. |
| Formulation: | Liquid. In PBS containing 0.05% sodium azide. |
| Handling: | Avoid freeze/thaw cycles. |
| Shipping: | Blue Ice |
| Long Term Storage: | -20°C |
| Scientific Background: | The ryanodine receptor (RyR) is the channel responsible for the release of calcium from the sarcoplasmic reticulum (SR) in muscle cells and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR-1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR-2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus). |
| Regulatory Status: | RUO - Research Use Only |
